A Novel Fluorescence Sensor for Highly Sensitive Detection of Glucose
10.11895/j.issn.0253-3820.160855
- VernacularTitle:高灵敏检测葡萄糖的新型荧光纳米传感器
- Author:
Aiqin LI
;
Chang GUO
;
Suying XU
- Keywords:
Organic fluorescent probes;
Fluorescence nanosensor;
Hydrogen peroxide;
Glucose;
Human serum
- From:
Chinese Journal of Analytical Chemistry
2017;45(6):824-829
- CountryChina
- Language:Chinese
-
Abstract:
A fluorescence nanosensor based on an easily prepared fluorescent molecule, 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD), was developed for highly sensitive detection of glucose.Under the catalysis of horseradish peroxidase (HRP), 3,3′,5,5′-tetramethylbenzidine (TMB) was oxidized into oxidized TMB (oxTMB) by H2O2.And the fluorescence of OPD was quenched by the intense absorption of the formed oxTMB, thus realizing effective quantitative detection of H2O2.The linear range was 0.05-0.8 μmol/L and 1-10 μmol/L respectively, with limit of detection of 0.02 μmol/L.Besides, on the basis of transformation of glucose into H2O2 through the catalysis of glucose oxidase, this nanosensor could be further exploited for highly sensitive detection of glucose.The TMB-HRP-OPD sensor exhibited linear range of 0.1-3.0 μmol/L and 4.0-30 μmol/L respectively for detection of glucose, with limit of detection of 0.02 μmol/L.Furthermore, it was successfully applied to the determination of glucose in real human serum and the results were in good agreement with the clinical data.
