Effects of Saikosaponin-d on Transcriptional Activation of Estrogen Receptor in Rat Hepatic Stellate Cells
10.13359/j.cnki.gzxbtcm.2017.04.019
- VernacularTitle:柴胡皂苷d对肝星状细胞内雌激素受体转录激活的影响
- Author:
Yirong CHEN
;
Renye QUE
;
Jinkai LIU
;
Yanting SHEN
;
Ni YAN
;
Yong LI
- Keywords:
saikosaponin-d/pharmacology;
hepatic stellate cells;
estrogen receptor;
luciferase reporter gene;
cell culture
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(4):550-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of saikosaponin-d on transcriptional activation of estrogen receptor in rat hepatic stellate cells(HSC-T6), and to explore its pharmacological mechanism. Methods The rat HSC-T6 were cultured in vitro for the study. After transient transfection of HSC-T6 with the ER-specific reporter gene ERE-tk-Luc by liposome, the effect of saikosaponin-d and estradiol on luciferase activity was observed by Dual-Luciferase Reporter Assay System under the conditions of various drug concentrations, various treatment time and addition of estrogen receptor inhibitor ICI182. 780. Results When the concentrations of saikosaponin-d and estradiol were in the range of 0.01-5 μmol/L, 0.01-1 μmol/L respectively, luciferase activity was increased in a dose-dependent manner . Luciferase activity arrived the highest when saikosaponin-d concentration was 5 μmol/L and estradiol concentration was 1 μmol/L, but the effect was weakening when estradiol concentration reached 5μmol/L. In respect of the effect of treatment time, when HSC-T6 were separately treated with 5μmol/L of saikosaponin-d and 1 μmol/L of estradiol for 24 h, the luciferase activity was the highest. And ICI182.780 could significantly inhibit the induction of saikosaponin-d and estradiol for luciferase activity. Conclusion Saikosaponin-d has an effect on promoting the transcriptional activation of estrogen receptor in HSC-T6.
