Content Determination of Ketoprofen in Ketoprofen Enteric-coated Capsules by Improving HPLC
10.6039/j.issn.1001-0408.2017.18.37
- VernacularTitle:改进HPLC法测定酮洛芬肠溶胶囊中酮洛芬的含量
- Author:
Xuxia ZHENG
;
Yuxue ZHOU
;
Peijian LIU
- Keywords:
Ketoprofen enteric-coated capsules;
Ketoprofen;
Content;
HPLC
- From:
China Pharmacy
2017;28(18):2579-2581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve HPLC for content determination of ketoprofen in Ketoprofen enteric-coated capsules. METHODS:HPLC method was adopted. The determination was performed on Chiralpak IC column with mobile phase consisted of n-hexane (0.1% TFA)-isopropanol(90:10,V/V)at a flow rate of 0.8 mL/min. The detection wavelength was set at 268 nm,and column temperature was 25 ℃. The sample size was 10 μL. RESULTS:The linear range of ketoprofen were 0.025-0.5 mg/mL(r=0.9998). The limit of quantitation was 1.0 mg/L,and limit of detection was 0.2 mg/L. RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recoveries were 96.36%-100.32%(RSD=1.87%,n=6). CONCLUSIONS:The method is sim-ple,accurate and rapid,and can be used for the content determination of ketoprofen in Ketoprofen enteric-coated capsules.
