Effect of Ningxia fruitless lycium sprout extracts on cardiomyocyte antioxidation and apoptotic protein expression in aging mice
10.3969/j.issn.1671-8348.2017.16.004
- VernacularTitle:宁夏无果枸杞芽水提物对老年小鼠心肌细胞抗氧化及凋亡蛋白表达的影响
- Author:
Xinwei MA
;
Lang LIU
;
Yikun CAO
;
Qin YAN
;
Yi QIN
;
Hua JIA
;
Lianxiang ZHANG
- Keywords:
aging;
aqueous extract of fruitless lycium sprout;
heart;
antioxidant;
apoptosis
- From:
Chongqing Medicine
2017;46(16):2170-2173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Ningxia aqueous extracts of fruitless lycium sprout (AEFLS) on cardiomyo cyte antioxidation and apoptosis-related protein expression in aging mice.Methods The natural aging C57BL/6J mice with 13 months old were randomly divided into aged control group,AEFLS low dose group (AEFLS1),AEFLS middle dose group (AEFLS2)and AEFLS high dose group(AEFLS3).The AEFLS1,AEFLS2 and AEFLS3 groups were respectively given with 5,10,20 mg/kg AEFLS gavage,while the aged control group was given with the normal saline gavage,for continuous8 weeks.The xan thine oxidase assay and thiobarbituric acid method were used for the determination of SOD and MDA in heart tissues.Western-blot and immunohistochemical method were used to detect the expressions of Bcl-2,Bax and Capase-3 in heart tissue.Results Compared with the aged control group,the MDA level in the AEFLS2 and AEFLS3 groups was decreased,while the SOD activity was increased,the difference was statistically significant (P＜0.01).The Western-blot result showed that compared with the aged control group,the optical density value of Bcl-2 in the AEFLS2 and AEFLS3 groups was increased,but the optical density values of Bax and Capase-3 were decreased (P＜0.01);the immunohistochemical results showed that compared with the aged control group,the immunopositive(IP) expressions of Bcl-2 protein in heart tissues in the AEFLS2 and AEFLS3 groups were increased (P＜0.01),while the IP expressions of Bax and Capase-3 were decreased (P＜0.01).Conclusion Middle and high doses of AEFLS can increase the antioxidative ability of myocardial tissue,up-regulates the Bcl-2 expression,down-regulates the Bax and Capase-3 expressions and plays anti-cardiomyocyte apoptotic role.
