Osteogenic ability of fascia-versus muscle-derived cells in rats
10.3969/j.issn.2095-4344.2017.16.003
- VernacularTitle:大鼠筋膜源性细胞与肌源性细胞成骨能力的对比研究
- Author:
Haibin SU
;
Guangheng LI
- From:
Chinese Journal of Tissue Engineering Research
2017;21(16):2472-2477
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Multipotent differentiation ability enables mesenchymal stem cells from autologous bone marrow to differentiate into osteoblasts and chondroblasts, thereby promoting the formation of bones and cartilage. However, the osteogenic ability differs from each other, and whose osteogenic ability is the best still needs to be studied further. OBJECTIVE: To compare the osteogenic ability of fascia- and muscle-derived stem cells in rats. METHODS: Fascia- and muscle-derived cells were isolated from 20 rats, followed by flow cytometry sorting, and were then cultured. FDC-LacZ cells were transfected with retro-BMP4 virus twice. Afterwards, the transfection efficiency of fascia-derived cells was detected through LacZ and alkaline phosphatase staining. RESULTS AND CONCLUSION: Compared with fascia-derived cells, muscle-derived cells showed stronger chondrogenic ability and produced more calcium deposition. These findings indicate that the osteogenic ability of muscle-derived cells is superior to that of fascia-derived cells in rats.
