Influence of different intensities of extracorporeal shock waves on the osteogenesis ability of alveolar osteoblasts
10.3969/j.issn.2095-4344.2017.16.009
- VernacularTitle:不同强度冲击波对牙槽骨细胞成骨能力的影响
- Author:
Yuan GUO
;
Chi ZHANG
;
Song LIU
;
Zhao WANG
;
Xingfei PAN
;
Le WANG
- From:
Chinese Journal of Tissue Engineering Research
2017;21(16):2509-2514
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Oral inflammatory diseases usually cause alveolar bone loss and odontoseisis, and further impact dental occlusion. Extracorporeal shock wave therapy (ESWT) is a promising method for the repair of alveolar bone and improving osteogenic activity of alveolar osteoblasts, but its therapeutic efficacy and related mechanisms remain unclear. OBJECTIVE: To compare the effect of different intensities of ESWT on the proliferation and osteogenesis abilities of rat alveolar osteoblasts. METHODS: The rat alveolar osteoblasts were obtained and cultured in vitro, and further identified by alkaline phosphatase staining. 0.18, 0.36, and 0.50 mJ/mm2 ESWT was used to stimulate the rat alveolar osteoblasts, 100 pulses, respectively. RESULTS AND CONCLUSION: The levels of alkaline phosphatase and bone morphogenetic protein 2 were significantly increased in the 0.36 and 0.18 mJ/mm2 ESWT groups, especially in the 0.36 mJ/mm2 ESWT group (P < 0.05). 0.50 mJ/mm2 ESWT significantly decreased the proliferation ability of rat alveolar osteoblasts and downregulated the levels of alkaline phosphatase and bone morphogenetic protein 2 (P < 0.05). To conclude, ESWT (< 0.36 mJ/mm2) can improve the osteogenesis ability of rat alveolar osteoblasts with the intensity increasing, which provides a theoretical basis for the clinical use of ESWT in the alveolar bone repair.
