Effect of Autophagy Inducer pp242 on Intestinal Barrier Dysfunction Induced by Tumor Necrosis Factor-α
10.3969/j.issn.1008-7125.2017.06.004
- VernacularTitle:自噬诱导剂pp242对肿瘤坏死因子-α所致肠屏障损伤的影响
- Author:
Cong ZHANG
;
Jiazheng WANG
;
Yujie SHEN
;
Mingxia ZHOU
;
Yingwei CHEN
- Keywords:
Autophagy;
Intestinal Mucosal Barrier;
Epithelial Cells;
Tumor Necrosis Factor-alpha;
Inflammatory Bowel Disease
- From:
Chinese Journal of Gastroenterology
2017;22(6):337-340
- CountryChina
- Language:Chinese
-
Abstract:
Intestinal barrier dysfunction and autophagy abnormality play important roles in the mechanism of inflammatory bowel disease, however, whether autophagy has effect on intestinal barrier dysfunction has not been reported.Aims: To explore the effect of autophagy inducer pp242 on intestinal barrier dysfunction induced by tumor necrosis factor-α (TNF-α).Methods: Model of intestinal epithelial monolayer barrier was established with Caco-2 cells in Transwell chambers, and then randomly divided into four groups: control group (without any intervention), TNF-α group (10 ng/mL TNF-α), pp242 group (1 μmol/L pp242), TNF-α+pp242 group (10 ng/mL TNF-α+1 μmol/L pp242).The intestinal barrier function was evaluated by transepithelial electrical resistance (TEER) and flux of FITC-dextran.The protein expressions of autophagy related protein LC3B-Ⅱ and p62 were detected by Western blotting.Results: Compared with control group, TEER was significantly decreased (P<0.05), flux of FITC-dextran, protein expressions of LC3B-Ⅱ and p62 were significantly increased in TNF-α group (P<0.05).Compared with TNF-α group, TEER was significantly increased (P<0.05), protein expression of LC3B-Ⅱ was significantly increased (P<0.05) while protein expression of p62 was significantly decreased in TNF-α+pp242 group (P<0.05).Conclusions: Autophagy inducer pp242 relieves TNF-α-induced intestinal epithelial barrier dysfunction via activating autophagy flux.
