Rapid Determination of Stilbene Glycoside in Shouwu Pills by Near Infrared Diffuse Reflectance Spectro-photometry
10.6039/j.issn.1001-0408.2017.18.26
- VernacularTitle:近红外漫反射光谱法快速测定首乌丸中二苯乙烯苷的含量
- Author:
Xile LIU
;
Canchao JIA
;
Shengguo JI
- Keywords:
Near infrared diffuse reflectance spectrophotometry;
Shouwu pills;
Stilbene glycoside;
Content;
Partial least square method
- From:
China Pharmacy
2017;28(18):2539-2542
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for the rapid determination of stilbene glycoside in Shouwu pills. METH-ODS:HPLC method was used to determine the content of stilbene glycoside in Shouwu pills(as measured value). The determina-tion was performed on ODS-C18 column with mobile phase consisted of acetonitrile-water(25:75,V/V)at the flow rate of 0.8 mL/min. The detection wavelength was set at 320 nm,and the column temperature was 30 ℃. The sample size was 10 μL. The partial least square method-near infrared diffuse reflectance spectrophotometry was used to establish quantitative calibration model for pre-dicting the content of stilbene glycoside in Shouwu pills. According to measured value,76 calibration set samples and 24 validation set samples were collected. Standard normalization method and first-order derivative method combined with Savitzky-Goly filter method were used for spectrum pretreatment. The optimal band ranged 9000-4150 cm-1,and main component factor was 12. RE-SULTS:The content determination method of stilbene glycoside in Shouwu pills was in line with the requirements. The correlation coefficients,the root-mean-square error of calibration,the root-mean-square error of predication and the root-mean-square error of cross-validation(RMSECV)of the quantitative calibration model were 0.99190,0.0201,0.0236 and 0.07629. There was no sta-tistical significance between predicted value and measured value(P>0.05). CONCLUSIONS:The method is accurate,stable and simple,and can be used for rapid determination of stilbene glycoside in Shouwu pills.
