Benzopyrene activates ERK1/2 signal pathway to promote extracellular matrix protein deposition of airway smooth muscle cells on airway remodeling
10.3969/j.issn.1671-8348.2017.16.005
- VernacularTitle:苯并芘激活ERK1/2信号通路促进气道平滑肌细胞外基质蛋白沉积对气道重塑的影响
- Author:
Yalu YUAN
;
Yuanxiong CHENG
;
Jing CAO
;
Wenyan LAI
;
Kaican CAI
- Keywords:
myocytes,smooth muscle;
extracellular matrix;
benzopyrene;
ERK1/2 pathway;
airway remodeling
- From:
Chongqing Medicine
2017;46(16):2174-2177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of benzopyrene on extracellular matrix(ECM) protein deposition of human airway smooth muscle cells(HASMC) and the related pathway mechanism.Methods HASMC were primarily cultured and the 2-6 generations cells were applied in this experiment.The expression amount of ECM gene and protein was detected by real time PCR and Western Blot the phosphorylation level was analyzed by using the Western Blot method.Results Benzopyrene could to increase the expression of HASMC collagen Ⅰ α1 protein (P＜0.01) and ECM protein (including collagen Ⅰ α1,versican,fibronectin,laminin α2) mRNA(P＜0.05).Benzopyrene could induce the rapid increase of ERK1/2 phosphorylation level (P＜0.01).Furthermore,the ERK pathway inhibitor PD98059 could significantly inhibit the increase of benzopyrene-induced collagen Ⅰ α1 (P＜0.01)and ECM protein(including collagen Ⅰ α1,versican,fibronectin,laminin α2) mRNA expression(P＜0.01).Conclusion Benzopyrene induces the ECM protein deposition of HASMC by activating the ERK1/2 pathway,blocking the ERK1/2 signal pathway can inhibit the benzopyrene-induced airway remodeling.
