Influence of esculentoside A on activation of ERK1/2-AP-1 pathway of glomerular mesangial cell induced by IL-1β
10.3969/j.issn.1671-8348.2017.16.007
- VernacularTitle:商陆皂苷甲对IL-1β诱导的肾小球系膜细胞ERK1/2-AP-1通路活化的影响
- Author:
Jieyin TANG
;
Yang DONG
;
Xianggui ZHANG
;
Han XIAO
;
Wei ZHANG
;
Lijun XU
- Keywords:
Esculentoside A;
cell proliferation;
glomerular mesangial cell;
AP-1;
ERK1/2
- From:
Chongqing Medicine
2017;46(16):2183-2186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of serum containing esculentoside A(EsA) on the proliferation of glomerular mesangial cell (GMC) and the activation of ERK1/2-AP-1 pathway of glomerular mesangial cell induced by IL-1β.Methods SD rats were gavaged by different doses of EsA(5,10,20,40 mg/kg) for getting medicated sera.The control group was set (gavage by 0.5sodium carboxymethylcellulose);the EsA medicated serum was used to treat rGMC.The control serum group was set.The influence of EsA medicated serum in each group on rGMC proliferation was detected by MTT;the rGMC was divided into the blank control group,IL-1β single action group,IL-1β+ EsA double action group,IL-1β+ U016 double action group and IL-1β+ U0126 + EsA combined action group,which were synchronized and then cultured for 48 h.Western blot was used to detectthe expression of p-ERK1/2 and AP-1 an the imaging analysis was performed.Results The EsA medicated serum(5-10 mg/kg gavage) inhibited the cellular proliferation(P＜0.05 or P＜0.01);the IL-1β group promoted the expression of p-ERK1/2 and AP-1 in rGMC(P＜ 0.05),after acting on rGMC in the IL-1β+EsA double action group,IL-1β+U0126 double action group and IL-1β+U0126+EsA combined action group,the expression of p-ERK1/2 and AP-1 was decreased(P＜0.05).Conclusion Serum containing EsA (5～10 mg/kg gavage) significantly inhibits the rGMC proliferation;EsA inhibits IL-1β induced rGMC proliferation,its action pathway on ERK1/2-AP-1 is one of mechanisms for inhibiting rGMC proliferation.
