Changes of platelet function in Alzheimer-like triple transgenic mice and its mechanism

VernacularTitle:阿尔茨海默病样三重转基因小鼠血小板的功能改变及机制探讨
Author: Yuanxin LIU ; Tao LIU
Keywords: 3xTg mice; Alzheimer's disease; Platelet function
From: Chinese Journal of Comparative Medicine 2017;27(6):17-21
CountryChina
Language:Chinese
Abstract: Objective To investigate the changes of the platelet function in APP/PS-1/tau(3xTg) mice, a murine model for Alzheimer''s disease,and explore its mechanisms.Methods We assessed the change of function of platelet in 3xTg-AD mice by flow cytometry.Adhesion assay and Western blotting were used to compare with the data of wild type mice.Results Platelets from aged 3xTg-AD mice were normal in number and glycoprotein expression (P>0.05), but adhere more avidly on matrices such as fibrinogen, compared with the platelets from age-matching wild type mice (P<0.05).The washed platelets of 3xTg-AD mice were adherent to fibrinogen, and also showed increased phosphorylation of selected signaling proteins, including PI3 kinase effector Akt and p38MAP kinase (P<0.05).In contrast, activation induced by several agonists in 3xTg-AD mice was similar to that of wild type platelets (P>0.05).Conclusions These results demonstrate that Alzheimer''s mutations result in a significant hyper-activated adhesion state of circulating platelets, evident with the progression of the disease.