Simultaneous Determination of Naringin and Neohesperidin in Weitong Pills by HPLC
- VernacularTitle:HPLC法同时测定胃痛丸中柚皮苷、新橙皮苷的含量
- Author:
Lingna PENG
;
Ye DING
;
Hui SUN
;
Wenli LI
;
Jiangnan LUO
- Keywords:
HPLC;
Weitong pills;
Naringin;
Neohesperidin
- From:
China Pharmacist
2017;20(6):1112-1114
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the content determination of naringin and neohesperidin in Weitong pills.Methods: An HPLC method was adopted.The determination was performed on an Agilent TC-C18 column with the mobile phase consisting of acetonitrile-water(20∶80)at the flow rate of 1.0 ml·min-1.The detection wavelength was set at 283nm.The column temperature was 30 ℃.Results: Naringin and neohesperidin respectively within the concentration range of 0.027-4.552 μg (r=0.999 9) and 0.029-4.016 μg(r=0.999 8) had good linear relationship with the peak area, the average recovery was 102.19% and 103.60%,and the RSD was 2.88% and 1.79%(n=9), respectively. Conclusion: The method is simple,accurate and reproducible, and suitable for the content determination of naringin and neohesperidin in Weitong pills.
