Simutaneous Determination of Three Anthraquinone Components in Knoxia Valerianoides by HPLC
- VernacularTitle:HPLC法同时测定云南红大戟中三种蒽醌类化合物的含量
- Author:
Chenjun WU
;
Jialin WANG
;
Jinpeng CHENG
;
Jing ZHAO
;
Yan LI
- Keywords:
Knoxia valerianoides;
HPLC;
Lucidin;
3-Hydroxymorindone;
Knoxiadin
- From:
China Pharmacist
2017;20(6):1120-1122
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC method for the simultaneous determination of 3 anthraquinones components in Knoxia valerianoides.Methods: The separation was performed on a Waters Xbridge C18column(250 mm×4.6 mm,5 μm), the mobile phase A was 0.05% phosphoric acid, and acetonitrile served as the mobile phase B, and the analysis was with gradient elution;the flow rate was 1.0 ml·min-1;the detection wavelength was 280 nm;the column temperature was 30℃.Results: The linearity of lucidin, 3-hydroxymorindone and knoxiadin was 0.147-29.400 μg·ml-1 (r=0.999 6), 0.126-25.200μg·ml-1 (r=0.999 9) and 0.135-27.000μg·ml-1 (r=0.999 5), and the average recovery was 98.50%(RSD=1.20%), 98.72%(RSD=0.73%) and 101.10%(RSD=1.12%)(n=6), respectively.Conclusion: The developed method for the simultaneous determination of 3 components can be used to control the quality of Yunnan Knoxia valerianoides.
