Inhibitory effect of ultrasound-mediated drug loaded carboxymethyl hexanoyl chitosan nanodroplet on ovarian cancer cells
10.13929/j.1003-3289.201612070
- VernacularTitle:超声介导正己酰羧甲基壳聚糖载药纳米液滴对卵巢癌细胞的抑制作用
- Author:
Muhua LUO
;
Bing SITU
;
Jianyi LIAO
;
Bingcheng LI
;
Zhiyi CHEN
- Keywords:
Carboxymethyl hexanoyl chitosan;
Drug loaded nanodroplet;
Ultrasonography;
Contrast media;
Ovarian neoplasms
- From:
Chinese Journal of Medical Imaging Technology
2017;33(6):838-842
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the inhibitory effect of phase change doxorubicin loaded carboxymethyl hexanoyl chitosan nanodroplet on ovarian cancer cells,and the effect of its ultrasound image in vitro.Methods The carboxymethyl hexanoyl chitosan synthesized through the acylationreaction with carboxymethyl chitosan and hexanoic anhydride.The drug loaded carboxymethyl hexanoyl chitosan nanodroplets were prepared by ultrasonic emulsification.The surface morphology,particle diameter and electric potential were characterized.Ultrasound imaging of the nanodroplet was evaluated in vitro.The encapsulation efficiency was determined by ultraviolet spectrophotometry.The survival rate of ovarian cancer cell was detected using CCK-8 reagent.The statistical analysis was performed.Results The drug loaded carboxymethyl hexanoyl chitosan nanodroplet was successfully prepared which showed regular morphology in microscope,the mean diameter of (458.33± 43.50)nm.The encapsulation efficiency was (52.06 ± 10.14)％.The nanodroplet could enhance ultrasonic imaging.The survival rate of ultrasound combined with drug loaded nanodroplet group ([62.54± 3.60]％) was lower than those of the free drug group ([75.55±7.21]％) and drug loaded nanodroplet group ([76.18±4.94]％),ultrasound group ([89.90±0.83]％;P＜0.05).Conclusion Ultrasound-mediated drug loaded nanodroplet can inhibit ovarian cancer cells,and has the potential for application in the clinical diagnosis and treatment.
