Study on chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine, L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry
10.7501/j.issn.1674-6376.2017.06.012
- VernacularTitle:D-赖氨酸、L-谷氨酰胺和L-酪氨酸对格列美脲及其顺式异构体识别的电喷雾质谱法研究
- Author:
Ying GENG
;
Caiyu ZHANG
;
Tianxing DAI
;
Lan HE
- Keywords:
cis/trans-isomer;
chiral amino acid;
electrospray ionization mass spectrometry;
glimepiride;
collision induced dissociation
- From:
Drug Evaluation Research
2017;40(6):792-796
- CountryChina
- Language:Chinese
-
Abstract:
Objective Chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine,L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry (ESI-MS) was achieved.Methods glimepiride/glimepiride-cis-isomer solutions were mixed with three amino acid solutions.The complex was extracted by ESI-MS and then the fragmentation abundance was investigated applying collision induced dissociation (CID) by MS/MS,which is the basis of chiral recognition for glimepiride and glimepiride-cis-isomer.Results Chiral recognition effect was achieved with the recognition rate (R) 1.61,2.92 and 2.17 for D-Lysine,L-Glutamine and L-Tyrosine respectively.Conclusion 3 kinds ofchiral amino acids were used as probes to distinguish between stereoisomers,and rapid identification of glimepiride and glimepiride cis isomer by mass spectrometry come true for the first time.
