HPLC Fingerprint study of Ziziphi Spinosae Folium
10.7501/j.issn.1674-6376.2017.06.013
- VernacularTitle:酸枣叶HPLC指纹图谱研究
- Author:
Xiang WANG
;
Chuang ZHANG
;
Shuaishuai FAN
;
Meng WANG
;
Liying NIU
- Keywords:
Ziziphi Spinosae Folium;
rutin;
fingerprint;
HPLC
- From:
Drug Evaluation Research
2017;40(6):797-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a specific HPLC method and a standard fingerprint for quality control ofZiziphi Spinosae Folium.Methods The samples were separated on a Kinetex C18 column (100 mm × 2.1 mm,2.6 μm) by gradient elution at the flow rate of 300 μL/min using acetonitrile and 0.1％ aqueous formic acid as the mobile phase.The column temperature was 30℃.The detection wavelength was 225 nm and the sample size was 10 μL.The fingerprint evaluation software (2012 edition) for Chinese materia medica (CMM) was used to evaluate the similarity of the 12 batches of samples.Results There were 13 characteristic peaks identified in the characteristic spectra ofZiziphi Spinosae Folium samples.Peak 6 was Rutin.The similarities of 13 batches ofZiziphi Spinosae Folium samples were proved to behigher than 0.850.Conclusion The method is available with a good reproducibility and accuraty which can control the quality standards effectively.
