Adhesion of Weissella cibaria to the Epithelial Cells and Factors Affecting its Adhesion.
10.4167/jbv.2006.36.3.151
- Author:
Mi Sun KANG
1
;
Mei Shan PIAO
;
Boo Ahn SHIN
;
Hyun Chul LEE
;
Jong Suk OH
Author Information
1. Department of Microbiology and Immunology, School of Medicine, Chonnam National University, Gwangju, Korea. joh@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Adhesion;
Weissella cibaria;
KB cell;
HeLa cell;
Fusobacterium nucleatum
- MeSH:
Adhesiveness;
Bacteria;
Epithelial Cells*;
Fusobacterium nucleatum;
HeLa Cells;
Humans;
KB Cells;
Lactic Acid;
Mouth;
Pronase;
Weissella*
- From:Journal of Bacteriology and Virology
2006;36(3):151-157
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We evaluated the ability of lactic acid bacteria, Weissella cibaria, isolated from the oral cavity to adhere to epithelial cells. W. cibaria efficiently adhered to KB cells and HeLa cells. In addition, W. cibaria efficiently adhered to Fusobacterium nucleatum. But the adhesiveness of W. cibaria disappeared upon exposure to LiCl or pronase, suggesting that the S-layer proteins of W. cibaria mediated the adhesiveness. The molecular mass of the S-layer proteins extracted from W. cibaria was approximately 50 kDa. When W. cibaria strains were washed with 0.45% saline, the bacteria were efficiently adhered to the epithelial cells. In conclusion, W. cibaria has the ability to adhere to epithelial cells through the S-layer proteins.
