Prepartation of Gelsolin-targeted ultrasound contrast agent and experiment in vitro

VernacularTitle:携Gelsolin单抗靶向超声造影剂的制备及体外靶向实验
Author: Haocheng QIN ; Jun WU ; Meng ZHOU ; Yuhong ZHANG ; Yu SONG ; Jieming LI ; Xiaona WEN ; Jianwu TANG ; Haitao RAN
Keywords: Ultrasonography; Contrast media; Target; Poly lactic-co-glycolic acid; Gelsolin
From: Chinese Journal of Medical Imaging Technology 2017;33(6):826-831
CountryChina
Language:Chinese
Abstract: Objective To prepare a kind of Gelsolin-targeted ultrasound contrast agent (GSN-PLGA) and to explore its targeting and imaging effection in vitro.Methods The high molecular PLGA-COOH ultrasound contrast agents were prepared by a modified double emulsion technique and then conjugated with Gelsolin monoclonal antibody by carbodiimide technique.The physical property of contrast agent was determined.And the connectivity condition of ultrasound contrast agent with Gelsolin monoclonal antibody was estimated.The targeting ability and the effect of enhancing ultrasound imaging in vitro were explored.Results The average diameter of GSN-PLGA was (575.67 ± 4.71) nm.The potential was (-11.46±1.19) mV.And the binding rate of Gelsolin monoclonal antibody was 96.93％.In vitro experimentshowed more GSN-PLGA could be intaked by Hca-F cells and the ultrasound imaging cloud be enhanced greatly.Conclusion The GSN-PLGA nanoparticle can bind to Hca-F cells specifically and can enhance the ultrasound imaging greatly.