Evaluation of ketamine-induced cerebral protection in mice with traumatic brain injury by magnetic resonance imaging
10.3760/cma.j.issn.0254-1416.2017.04.032
- VernacularTitle:MRI法评价氯胺酮对创伤性脑外伤小鼠的脑保护作用
- Author:
Xuan GAO
;
Fang FANG
;
Xiaomin LING
;
Ruixue SONG
;
Mengyuan PENG
;
Zhanggang XUE
;
Jing CANG
- Keywords:
Ketamine;
Brain injuries;
Magnetic resonance imaging
- From:
Chinese Journal of Anesthesiology
2017;37(4):501-503
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate ketamine-induced cerebral protection in mice with traumatic brain injury (TBI) by magnetic resonance imaging (MRI).Methods Thirty-two pathogen-free healthy male C57BL/6 mice,aged 8 weeks,weighing 26-30 g,were divided into 4 groups using a random number table:control group (group C,n=7),ketanine group (group K,n=7),TBI group (n=9) and TBI plus ketamine group (group TBI+K,n =9).TBI was produced with a pneumatically driven controlled cortical impact device.Ketamine 150 mg/kg was intraperitoneally injected at l h after operation in TBI+K and K groups,while the equal volume of normal saline was given instead in TBI and C groups.Open field test was conducted at 24 h,72 h and 7 days after operation.The animals in TBI and TBI+K groups were scanned by T1-weighted MRI at 6,24 and 72 h after operation,the animals in C and K groups were scanned by MRI at 24 h after operation,and the development of cerebral edema was observed.Results MRI scan showed no cerebral edema in C and K groups,and different degrees of cerebral edema were found in TBI and TBI+K groups.Compared with group C,the locomotor distance was significantly shortened at 24 and 72 h after operation in group TBI (P＜0.05).Compared with group TBI,the size of cerebral edema was significantly decreased,and the locomotor distance was prolonged at 24 and 72 h after operation in group TBI+K (P＜0.05 or 0.01).Conclusion MRI method further clarifies that ketamine can produce cerebral protection to some extent in mice with TBI.
