Effect of different formula diets on mouse model with DEN-induced hepatocellular carcinoma
10.3969/j.issn.1005-4847.2017.03.013
- VernacularTitle:不同配方饲料对DEN诱导小鼠肝癌模型建立的影响
- Author:
Xiaodong HE
;
Yihe GUI
;
Binglin LI
;
Huijun DENG
;
Jiangchao LI
;
Lijing WANG
- Keywords:
Diet;
Diethylnitrosamine;
Hepatocellular carcinoma;
Mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(3):306-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a mouse model of diethylnitrosamine(DEN)-induced hepatocellular carcinoma (HCC),and to explore the effects of two different diet formulas on the establishment of DEN-induced HCC model.Methods SPF C57BL/6 mice (8 males and 8 females) were injected intraperitoneally with 25 mg/kg DEN at day 14 to establish a HCC model.The mice were divided into two groups after weaning.One group was fed with the SPF class rodents cereal-based diet,another group was fed with AIN-93G formula diet.The mice were sacrificed at the age of 9 months.The livers were weighed and the growth of liver cancer was observed and recorded.Results All the mice in the cereal-based diet group developed HCC as expected.The body weight and liver mass of the mice in the AIN-93G diet group were significantly lower than that of the cereal-based diet group.The incidence of HCC,and the number and size of tumor nodules were also significantly lower in the AIN-93G diet group than that in the cereal-based diet group.Conclusions DEN-induced HCC model has been successfully established in mice fed with cereal-based diet,while mice fed with AIN93-G diet prevented the development of DEN-induced HCC,and their body weight was decreased significantly,suggesting that dietary factors play a key role in establishment of animal disease models.
