Correlation analysis of human papilloma virus 16/18 infection and the expression of Rb and p16 protein in bladder cancer tissue
10.3969/j.issn.1673-4130.2017.12.019
- VernacularTitle:人乳头瘤病毒16/18感染与膀胱癌组织中Rb和p16蛋白表达的相关性分析
- Author:
Zhengrong ZHANG
;
Junxiang DU
;
Yun XIE
;
Qun XIE
;
Junyong CHEN
;
Qiwei NIE
- Keywords:
human papilloma virus;
bladder cancer;
Rb protein;
p16 protein
- From:
International Journal of Laboratory Medicine
2017;38(12):1636-1638
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between human papilloma virus(HPV) 16/18 infection and the expression of Rb and p16 protein in bladder cancer tissue,and to analyze the relationship between HPV infection and the incidence of bladder cancer.Methods The expression of HPV16/18 E6 and E7 gene encoded protein,RB and p16 were detected by immunohistochemical method in 40 cases of bladder cancer and 40 cases of normal bladder tissues,and the correlation between them and pathological grading,stage of international union of cancer(UICC),whether recurrence or not after receiving surgery was analyzed.Results In bladder cancer tissues,HPV16/18 E6 and E7 gene encoded protein,RB and p16 positive rates were 65%,47.5%,42.5%,compared with the positive rate of normal bladder tissue samples(22.5%,92.5%,87.5%),the differences were statistically significant(P<0.05).The expression of HPV16/18 E6 and E7 gene encoded protein,Rb and p16 proteins were associated with pathological grading and staging of bladder cancer(P<0.05),but were not related to the tumor recurrence(P>0.05).The expression of HPV16/18 E6 and E7 gene encoded protein and Rb,p16 protein were not significantly correlated(P>0.05),The expression of Rb and p16 protein were negatively correlated(P<0.05).Conclusion HPV 16/18 infection is related to the occurrence and development of bladder cancer,but its mechanism might not be related to the abnormal expression of Rb and p16 protein.
