Suppression of RANKL/OPG pathway activation in FLSs in interfacial membrane through up-regulation of HIF-1α induced by Ti particles
10.3969/j.issn.1006-5725.2017.10.013
- VernacularTitle:钛颗粒诱导松动界膜中滑膜成纤维细胞HIF-1α的表达抑制其对RANKL/OPG信号通路的活化作用
- Author:
Chengqiang WANG
;
Shengmao HE
;
Xiaobo XIE
;
Qi LI
- Keywords:
Aseptic loosening;
Fibroblast-like synoviocytes;
HIF-1α;
RANKL/OPG;
Ti particles
- From:
The Journal of Practical Medicine
2017;33(10):1580-1583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between HIF-1α expression and RANKL/OPG pathway activation of fibroblast-like synoviocytes(FLSs)induced by Ti particles in aseptic loosening interfacial membrane. Method FLSs were extracted from the synovial tissue collected in surgeries and then co-cultured with Ti particles. QRT-PCR and Western blotting were conducted to measure the mRNA and protein expression of RANKL ,OPG and HIF-1αin FLSs at different concentration and time. Results Genes and protein expression levels of RANKL/OPG and HIF-1α were up-regulated with the increase of concentration of Ti particles. Expression of HIF-1α gene and protein increased time-dependently;the mRNA and protein expression of RANKL/OPG increased firstly and then declined alongside the increase of HIF-1αexpression. Conclusions Ti particles induce the up-regulation of HIF-1α expression and activate RANKL/OPG pathway. Up-regulation of HIF-1α may suppress the activation of RANKL/OPG pathway in FLSs of the interfacial membrane induced by Ti particles.
