Induced Pluripotent Stem Cells-derived chimeric Thymus transplantation enhances T cells Reconstitution and prevents GVHD in Murine after allogeneic Bone Marrow Transplantation
10.3969/j.issn.1006-5725.2017.09.013
- VernacularTitle:iPS细胞来源的嵌合体胸腺移植促进异基因骨髓移植小鼠的T细胞重建和抑制移植物抗宿主病
- Author:
Wenling GUO
;
Cuiling WU
;
Hui LIANG
;
Ming SHI
;
Yuming ZHANG
- Keywords:
Induced pluripotent stem cell;
Allogeneic bone marrow transplantation;
Thymus transplan-tation;
T cells reconstitution;
Graft versus host disease
- From:
The Journal of Practical Medicine
2017;33(9):1414-1418
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of iPS cells-derived chimeric thymus transplantation on T cells reconstitution and graft versus host disease of murine after allo-BMT. Methods iPS cells-derived chimeric thymus was grafted under the renal capsules of mice after allogeneic IBM-BMT. The mice were divided into three groups:IBM-BMT group, IBM-BMT+TT group and IBM-BMT+DLI group. Four weeks after BMT, T lymphocyte subsets in the peripheral blood were analyzed by flow cytometry, the degree and pathological examination of GVHD were observed, respectively. Results Percentage of CD8+T cells in IBM-BMT group, IBM-BMT+TT group and IBM-BMT+DLI group was(5.52 ± 0.83)%,(11.10 ± 1.49)%and(8.49 ± 0.82)%respectively, there was signifi-cant difference between pairwise comparisons(P<0.05), and percentage of CD4 + T cells of the peripheral blood in IBM-BMT+TT group(9.60 ± 0.69)%was significantly higher than IBM-BMT group(6.42 ± 1.40)%and IBM-BMT+DLI group(8.07 ± 0.65)%(P<0.05) . IBM-BMT group and IBM-BMT+TT group showed less clinical and histopathological scoring of GVHD than IBM-BMT + DLI group. Conclusion iPS cells-derived chimeric thymus transplantation could effectively accelerate T cells reconstitution and prevent GVHD after allo-BMT.
