Antibiotic susceptibility and class I integron analysis of imipenem-resistant Pseudomonas aeruginosa

VernacularTitle:亚胺培南耐药铜绿假单胞菌I类整合子检测
Author: Huiqin TANG ; Dingqiang CHEN ; Aiwu WU
Keywords: Pseudomonas aeruginosa; Class I integron; Drug resistance gene cassette
From: The Journal of Practical Medicine 2017;33(9):1494-1497
CountryChina
Language:Chinese
Abstract: Objective To investigate the class I integrons and their gene cassettes of imipenem-resistant Pseudomonas aeruginosa (IRPA) , and to analyze the correlation between integrons and drug resistance. Methods PCR was used to determine the presence of integrase genes and class I integrons. The variable regions were detected by sequencing. Resistance genes of integron gene cassettes including metal-β-lactamases, aminoglycoside modifying enzymes (AMEs), 16SrRNA methylating enzyme and the OprD2 genes were detected by PCR. The VITEK-2 automated system was used to determine the antibiotic susceptibility of integron-positive IRPA strains. Results The positive rates of integrase genes and class I integrons were 23.3%(20/86)and 8.14%(7/86) , and five kinds of gene cassettes were detected in 86 IRPA strains. The class I integrons-positive bacterial strains exhibited different resistant patterns to 12 antibiotics with large number of resistance genes. Conclusion The class I integrons and their gene cassettes are associated with multiple drug resistance of IRPA.