Influence of EMT in Radiosensitivity of PC-9:an EGFR- mutant NSCLC cell line
10.3969/j.issn.1006-5725.2017.10.012
- VernacularTitle:上皮-间质转化对EGFR突变型非小细胞肺癌细胞株PC-9放射敏感性的影响
- Author:
Zhihao ZHANG
;
Juan ZHOU
;
Weimin ZHANG
- Keywords:
Non-small cell lung cancer;
Epithelial mesenchymal transition;
EGFR-mutant;
Radio-sensitivity
- From:
The Journal of Practical Medicine
2017;33(10):1576-1580
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of epithelial mesenchymal transition(EMT)in radiosensi-tivity of EGFR-mutant NSCLC cell with acquired resistance to EGFR-TKI. Methods In this study,EGFR-mutant human lung adenocarcinoma cell line PC-9 and Gefitinib acquired resistance cell line PC-9/AB were used. Western blot was used to assess EMT. Wound healing migration assay was tested. CCK8,colony formation and flow cytome-try were used to evaluate survival fraction ,as the sensitivity to irradiation. Results PC-9/AB displayed radioresis-tance(P<0.05). When EMT was reversed with CDH1,its radiosensitivity was significantly higher than PC-9/AB (P < 0.05). PC-9/TGF also displayed radioresistance (P < 0.05),as well as EMT phenotype presented. Conclusion EMT enhanced the radioresistance of EGFR-mutant NSCLC cell with acquired resistance to EGFR-TKI, possibly through TGF-βpathway.
