Effect of Yiqixue Recipe on Ovary Apoptosis and Expression of Bim in Ovarian Granulosa Cells of Con-trolled Ovarian Hyperstimulation Rats
10.3969/j.issn.1006-5725.2017.10.016
- VernacularTitle:益气血法对超排卵大鼠卵巢颗粒细胞凋亡及Bim表达的影响
- Author:
Zhen YANG
;
Xinyu GUO
;
Weimin DENG
;
Jinyu ZHANG
;
Renfei YUAN
;
Lulu TANG
- Keywords:
Yiqixue recipe;
Jinghou Zengzhi Recipe;
Controlled ovarian hyperstimulation;
granulosa cells apoptosis;
Bim
- From:
The Journal of Practical Medicine
2017;33(10):1593-1596
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effects of Jinghou Zengzhi Recipe(JHZZR),a Chinese prescription with the action of tonifying Qi and blood ,on the ovary apoptosis and expression of Bim in ovarian granulosa cells (GCs) of controlled ovarian hyperstimulation(COH) rats , and analyze the possible therapeutic mechanism. Methods A model of COH rats were prepaered and 30 female SD rats were randomly divided into 5 groups , including control group,positive control group,low,medium and high concentration group in six rats in each group. The apoptosis index(AI)in ovarian GCs were detected by TUNEL ,and the expression of Bim by qPCR. Results The AI of ovarian GCs in high and medium concentration group were obviously lower(P < 0.05)than that in the positive control group,and there is no significant differences(P > 0.05)comparing with control group. The mRNA levels of Bim were lower(P<0.05)in all concentration group than those in positive control group ,but still higher(P<0.05)than that in control group. And there is no significant differences(P>0.05)during the three concentration groups in Bim mRNA. Conclusion JHZZR can inhibit the ovarian GCs apoptosis of COH rats through decreasing the expression of Bim mRNA ,which improve the quality of ovarian follicle.
