The mechanism research of STIM1 in breast cancer cells
10.3969/j.issn.1006-5725.2017.09.003
- VernacularTitle:STIM1对乳腺癌细胞移动的作用及机制
- Author:
Bing WU
;
Tianji LIN
;
Shijuan RUAN
;
Bin WANG
;
Fei ZOU
- Keywords:
Breast cancer cells;
STIM1;
Calcium signaling;
FAK
- From:
The Journal of Practical Medicine
2017;33(9):1373-1376
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To explore the calcium signaling mechanism of STIM1 in breast cancer cells. Meth-ods After SiRNA interruption, Western blot and Transwell were used to measure protein expression of STIM1 and cell migration in MDA-MB-231 cells respectively. The relationship between STIM1 and SOCE calcium signaling were analysed by Laser confocal microscopy. Western blots were used to measure protein expression of FAK after si-lence STIM1. Results The numbers of cells without STIM1 were significantly lower than those cells with STIM1 by Transwell assay. STIM1 mediated SOCE in MDA-MB-231. Blocking SOCE might inhibite cells migration. Si-lence STIM1 did not affect the expression or activation of FAK in MDA-MB-231 cells. Conclusion STIM1 influ-ences cell migration through SOCE pathway in breast cancer cells, which is independent on the expression or activa-tion of FAK.
