Inhibition effect of curcumin on proliferation and invasion of breast cancer cell by decreasing expression of MT1-MMP

VernacularTitle:姜黄素通过降低MT1-MMP表达抑制乳腺癌细胞的增殖与侵袭
Author: Junqing YU ; Guangyu YAO ; Xiaolei HU ; Lujia CHEN ; Changsheng YE
Keywords: Breast neoplasm; Curcumin; MCF-7 cell; MT1-MMP
From: The Journal of Practical Medicine 2017;33(9):1394-1396
CountryChina
Language:Chinese
Abstract: Objective To investigate whether MT1-MMP is involved in the inhibition effect of curcumin on the proliferation and invasion of breast cancer cell and the mechanism . Methods Firstly, MCF-7 cell lines transfected by MT1-MMP eukaryotic expression vector was established. We divided all cells into 3 groups,including null vector transfection group, non-transfected and transfected group with different concentrations of curcumin. The expression of MT1-MMP protein, the proliferation and invasion ability were respectively analyzed by western blot, transwell method, and cell counting kit-8 (CCK-8). Results The expression of MT1-MMP was inhibited by curcumin. Transwell and CCK-8 experiment indicated the proliferation and invasion abilities of MT1-MMP transfected MCF-7 cells were inhibited by curcumin in a concentration dependent manner. Conclusion The inhibition value of curcumin on proliferation and invasion is probably due to its ability to inhibit the expression of MT1-MMP.