Proliferation and migration of breast cancer cells inhibited by miRNA-16 in vitro Bu Deyong , Jia Hongyan
10.3760/cma.j.issn.1006-9801.2017.06.002
- VernacularTitle:miRNA-16抑制乳腺癌细胞增殖和迁移的体外研究
- Author:
Deyong BU
;
Hongyan JIA
- Keywords:
Breast neoplasms;
Cell proliferation;
Cell movement;
MicroRNA 16
- From:
Cancer Research and Clinic
2017;29(6):366-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miRNA-16 (miR-16) in breast cancer patients and its effect on the proliferation and migration of breast cancer cells. Methods Polymerase chain reaction (PCR) was used to detect the expression of miR-16 in 30 breast cancer patients. miR-16 mimics was transfected to MDA-MB-231 and MCF-7 cell, and CCK-8 as well as Transwell assay was applied to detect the effect of miR-16 on cell proliferation and migration of MDA-MB-231 and MCF-7 cells. Results Among 30 breast cancer patients, the expression of miR-16 was down-regulated in 23 cases. Cell proliferation and migration of MDA-MB-231 and MCF-7 cells were inhibited significantly after transfection of miR-16 mimics. Conclusion miR-16 is down-regulated in breast cancer patients. miR-16 inhibits significantly the cell proliferation and migration of breast cancer cells in vitro.
