Expression and significance of EZH2 protein in primary testicular diffuse large B cell lymphoma
10.13315/j.cnki.cjcep.2017.05.004
- VernacularTitle:睾丸原发性弥漫大B细胞淋巴瘤中EZH2蛋白的表达及其意义
- Author:
Shirong MA
;
Yingmei WANG
;
Yang LIU
;
Shuangping GUO
- Keywords:
primary testicular diffuse large B cell lymphoma;
EZH2;
clinicopathological feature;
immunophenotype;
prognosis
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(5):485-489
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the expression and significance of histone methyltransferase EZH2,clinicopathological features in primary testicular diffuse large B-cell lymphoma (DLBCL).Methods Immunohistochemical of Ventana Ultra View two-step staining was used to detect expression of EZH2 in 17 cases of primary testicular DLBCL.The relationship between EZH2 expression and its clinicopathological features were analyzed.Sanger squencing was used to detect EZH2 Y641 mutation in these cases.Results Morphologically,the tumor cells resembled centroblasts in 11 cases,immunoblasts in 3 cases,and anaplastic variants in 3 cases.Immunophenotypically,14 cases were non-germinal centre B cell like (non-GCB) type and 3 cases were germinal centre B cell like (GCB) subtype.EZH2 overexpressed in all 17 cases.EZH2 overexpressed in nearly tumor celts with uniformly strong intensity in 15 cases,and more than 70％ tumor cells with moderate to strong intensity in 2 cases.The follow-up information was obtained in 9 patients,with a median survival time of 35 months.No association was found between the level of EZH2 expression and outcome of patients.No mutation of EZH2 Y641 was detected.Conclusion Primary testicular DLBCL is a rare aggressive B cell lymphoma with distinctive clinicopathological features.Detection of EZH2 expected to assist in the diagnosis and treatment of tumor.
