Expression of IgH/CCND1 fusion gene and cell cycle associated protein in mantle cell lymphoma
10.13315/j.cnki.cjcep.2017.05.009
- VernacularTitle:套细胞淋巴瘤中IgH/CCND1融合基因及细胞周期相关蛋白的表达
- Author:
Shunping CHEN
;
Wenqiao WU
;
Hongwu SHEN
;
Zongkai ZOU
;
Haiyan SU
;
Siling JI
;
Shaojun HONG
- Keywords:
mantle cell lymphoma;
CCND1;
fusion gene;
fluorescence in situ hybridization
- From:
Chinese Journal of Clinical and Experimental Pathology
2017;33(5):511-514
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of cell cycle related protein including Cyclin D1,CDK4,p16 and IgH/CCND1 fusion gene in mantle cell lymphoma (MCL) and their relationship with each other.Methods The expression of cell cycle related protein including Cyclin D1,CDK4,p16 and IgH/CCND1 fusion gene were detected on the 40 cases of MCL (expreimental group) and 20 cases of reactive hyperplasia (control group) by using the combined detection of fluorescence in situ hybridization (FISH) and immunohistochemistry of EnVision two methods.40 cases of MCL were confirmend by using gene rearrangement technique and immunohistochemistry.The threshold of IgH/CCND1 fusion gene of MCL was established in the control group.Results In the experimental group,Cyclin D1 protein positive expression rate was 100％,the positive expression of CDK4 protein rate was 87.50％,p16 protein positive expression rate was 17.50％.Positive rate of IgH/CCND1 fusion gene of 100％.These cell cycle related protein and IgH/CCND1 fusion gene were negative in the control group.Conclusion In MCL,Cyclin D1-CDK4-p16 pathway is consistent with the principle of tumor cell cycle regulation.The establishment of threshold value of IgH/CCND1 fusion gene by FISH technique may provide the basis for the judgement of FISH of the IgH/CCND1 in China.
