The expression of Sema3a/Nrp1 signal axis in the periodontal tissue with chronic periodontitis and it's role in bone destruction
10.3969/j.issn.1001-3733.2017.02.001
- VernacularTitle:Sema3A/Nrp1信号轴在慢性牙周炎牙周组织中的表达及在牙周骨质破坏中的作用
- Author:
Ying LIN
;
Quan XING
;
Xianling GAO
;
Meng XU
;
Zhengmei LIN
- Keywords:
Chronic periodontitis(CP);
Bone destruction;
Sema3a/Nrp1 signal axis
- From:
Journal of Practical Stomatology
2017;33(2):143-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of Semaphorins 3A(Sema 3A) and its receptor Neuropilin-1 (Nrp1) in the development of chronic periodontitis of rats and clinical samples.Methods:20 SD rats were divided into 2 groups.Rats in the experimental group were induced into chronic periodontitis models.Rats in control group were not treated.After 8 weeks,maxilla of all the rats were collected for micro-CT scanning and IHC staining.The distance from cementoenamel junction to alveolar bone crest(CEJ-ABC) and the IOD of Sema3A/Nrp1 positive staining in rats were analyzed.20 clinical samples of chronic periodontitis(n =10) and normal periodontal tissues (n =10) were collected for immunofluorescence and qRT-PCR analysis.Results:The CEJ-ABC distance of chronic periodontitis group was higher than that of the control group(P ＜ 0.05).The IOD of Sema3A/Nrp1 in experimental group was lower than that of the control group (P ＜ 0.05) and with a negative correlation with bone loss (P ＜ 0.05).Immunofluorescence and qRT-PCR analysis showed that the expression level of Sema3a/Nrpl in clinical samples with chronic periodontitis was also lower than that of the healthy subjects(P ＜ 0.05).Conclusion:The reduced Sema3A/Nrp1 plays an important role in the development of bone destruction in chronic periodontitis.
