Bone regeneration with bone marrow mesenchymal stem cells, platelet rich plasma and decellularized cartilage matrices complex
10.3969/j.issn.1001-3733.2017.02.002
- VernacularTitle:骨髓间充质干细胞复合脱细胞软骨和富血小板血浆的体内成骨实验
- Author:
Xiaoke FENG
;
Yan DONG
;
Zhiye LI
;
Wei WU
;
Yimin ZHAO
- Keywords:
Bone marrow mesenchymal stem cells (BMSCs);
Platelet rich plasma(PRP);
Decellularized cartilage matrix (DCM);
Endochondral ossification
- From:
Journal of Practical Stomatology
2017;33(2):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the bone regeneration capacity of the complex of bone marrow mesenchymal stem cells(BMSCs) and platelet rich plasma (PRP) with decellularized cartilage matrix (DCM).Methods:BMSCs were isolated from young rabbit and cultured;PRP were prepared from the fresh blood of rabbit and the DCM were come from the fresh ears of rabbits and processed into a mixture of BMSCs-PRP-DCM.The mixture was injected subcutaneously into nude mice,8 weeks after injection bone regeneration was examine by HE staining and Massons staining decellularization.Results:The BMSCs show good differentiation capacity in vitro and the cartilage fragments were well decellularized.Excellent endochondral ossification ability of the complex was observed by in vivo experiment.Conclusion:The BMSCs-PRP-DCM complex has good capacity of endochondral ossification.
