The effects of BMP9 combined with NGF on the osteogenic differentiation of C3H10T1/2 mesenchymal stem cells
10.3969/j.issn.1001-3733.2017.02.003
- VernacularTitle:BMP9联合NGF对C3H10T1/2间充质干细胞骨向分化能力影响的实验研究
- Author:
Junyu LIU
;
Kun YANG
;
Gang LI
;
Yingying WANG
;
Xiujie WEN
;
Yinghui TAN
- Keywords:
Bone morphogenetic protein 9 (BMP9);
Nerve growth factor (NGF);
Mesenchymal stem cells (MSCs);
Osteogenic differentiation
- From:
Journal of Practical Stomatology
2017;33(2):152-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of BMP9 combined with NGF on the osteogenic differentiation of mesenchymal stem cells(MSCs).Methods:Recombinant BMP9 adenovirus was transfected into C3H10T1/2 cells.The cells were treated by GFP,NGF,BMP9 and BMP9 + NGF respectively.The expression level of COL1,RUNX2 was detected by RT-PCR and Western blot,ALP activity was examined by ALP kit 3,12,24,48 hours,3 and 7 days after treatment,respectively.Results:The ALP activity of BMP9 + NGF group was the highest among the 4 groups.The difference in the groups firstly appeared at 3 h after treatment.The highest expression level of RUNX2 and COL1 was detected in BMP9 + NGF group.Conclusion:NGF and BMP9 may synergisticly promote osteogenic differentiation at the early stage of osteogenic induction of C3H10T1/2 cells.
