Comparison of the biological safety of three metal dental materials
10.3969/j.issn.1001-3733.2017.02.008
- VernacularTitle:3种应用于正畸支抗的金属材料的细胞毒性和遗传毒性检测
- Author:
Qinghua ZHANG
;
Hong FAN
- Keywords:
Pure Ti;
Ti alloy;
Stainless steel;
Biological safety
- From:
Journal of Practical Stomatology
2017;33(2):179-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the biological safety of commercial pure Ti (cpTi),Ti alloy Ti6Al4V and stainless steel 316L.Methods:According to the GB/T16886.5-2003 and YY/T0127.2-2009 standards,the extraction of the 3 materials was respectively prepared,cck-8 assay was used to test their cytotoxicity,single cell gel electrophoresis was used to test their genotoxicity,and in vivo test was used to test their acute systemic toxicity.Results:The cytotoxicity of 100％ extractions of 316L,Ti6Al4V and cpTi was graded to 2,1 and 0,the olive tal moment(OTM) 10.79 ±0.39,9.84 ± 1.78 and 0.92 ± 1.43,the percentage of tail DNA (1.22 ±0.06) ％,(0.92 ± 0.43) ％ and (0.43 ± 0.01) ％ respectively.No systemic toxicity and no body weight change was observed in the acute systemic toxicity test in mice.Conclusion:cpTi has better biologic safety than Ti6Al4V and 316L.
