c-fos modulates p16/CyclinD1 signaling pathways and promotes the proliferation and migration of oral squamous cell carcinoma
10.3969/j.issn.1001-3733.2017.01.022
- VernacularTitle:c-fos调控p16/CyclinD1信号途径并促进口腔鳞状细胞癌增殖和迁移
- Author:
Rui LUO
;
Ruizhi ZHANG
;
Zhenglin GONG
;
Ping ZHANG
- Keywords:
c-fos;
CyclinD1;
Oral squamous cell carcinoma;
Proliferation;
Migration
- From:
Journal of Practical Stomatology
2017;33(1):100-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of c-fos on the proliferation and migration of oral squamous cell carcinoma and potential mechansism.Methods:The expression of c-fos,CyclinD1 and p16 in 60 oral squamous cell carcinoma samples and 60 oral mucosa tissue samples was examined by immunohistochemistry.HN6 and SCC9 cells were respectively transfected with siRNA-c-fos and siRNA-scramble,then were respectively divided into control group,siRNA-scramble group and siRNA-c-fos group.The mRNA and protein expressions of CyclinD1 and p16 were decteted,meanwhile cell proliferation and migration were tested.Results:Compared with the oral mucosa tissue samples,the expressions of CyclinD1 and c-fos were increased in the carcinoma samples,while the expression of p16 was reduced.Compared with control group,the expressions of CyclinD1 in siRNA-c-fos group were significantly reduced,while p16 enpression was increased,with the inhibition of cell proliferation and migration.Conclusion:c-fos may regulate pl6/CyclinD1 signaling pathways and promote the proliferation and migration of oral squamous cell carcinoma.
