Preparation and characterization of chitosan/hyaluronic acid nanoparticles for microRNA-148b delivery
10.3969/j.issn.1001-3733.2017.01.007
- VernacularTitle:载microRNA-148b壳聚糖/透明质酸纳米粒制备及鉴定
- Author:
Guangsheng WU
;
Guangyan HUI
- Keywords:
microRNA;
Chitosan;
Nanoparticle;
Mesenchymal stem cells (MSCs)
- From:
Journal of Practical Stomatology
2017;33(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare and characterize chitosan/hyaluronic acid (CS/HA) nanoparticles for miRNA-148b (miR-148b)delivery.Methods:CS/HA/miR-148b nanoparticles were prepared.The particle size,zeta potential,morphology were investigated by nano laser granulometer and TEM respectively.The encapsulation efficiency of CS/HA nanoparticles was evaluated by gel retarding analysis.The cytotoxicity and transfection efficiency of CS/HA/miR-148b nanoparticles on rat bone marrow mesenchymal stem cells (MSCs) were evaluated by CCK-8 assay,transfection assay and FCM respectively.Results:The CS/HA/miR-148b nanoparticles were discrete spherical particles with the diameter of 160 to 370 nm and zeta potential of + 15 to +41 mV.N/P ratio at 20∶1 of the nanoparticles showed the optimum encapsulation efficiency for miR-148b.CS/HA/miR-148b nanoparticles exhibited no cytotoxicity to MSCs and could deliver miR-148b to the MSCs at high transfection efficiency.Conclusion:CS/HA/miR-148b nanoparticles are safe and effective for miR-148b delivery into MSCs.