The effects of environmental changes on E.faecalis growth and penetration into microtubes in a microfluid chip
10.3969/j.issn.1001-3733.2017.01.011
- VernacularTitle:营养缺乏和碱性环境对粪肠球菌侵入人工微管系统的影响
- Author:
Xiaoqiang SUN
;
Shujing WANG
;
Chunxiong LUO
;
Benxiang HOU
- Keywords:
Dentinal tubules;
Enterococcus faecalis;
Microfluidics;
Persistent apical periodontitis
- From:
Journal of Practical Stomatology
2017;33(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of different growth conditions on E.faecalis growth in the microfluid chip and the penetration of E.faecalis into microtubes.Methods:Four units on the chip were randomly selected as control,BHI,nutrient-Tdeprived (PBS) and pH 10 groups.The growth of E.faecalis was monitored by microscope for a period of 72 h after the suspension of E.faecalis had been added into the chip.Results:The microscopic analysis showed a distinct variation in the growth rate and morphological feature under different experimental conditions.he depth of bacterial penetration was significantly greater in BHI group.Conclusion:This study demonstrated that environmental changes can significantly influence the growth and penetration of E.faecalis into the micro tubes.
