Genotyping and gene polymorphism of Neisseria gonorrhoeae with azithromycin-resistance and decreased susceptibilities to ceftriaxone
10.3969/j.issn.1673-4130.2017.11.020
- VernacularTitle:阿奇霉素耐药及头孢曲松低敏淋球菌基因分型与多态性研究
- Author:
Xiaodong LI
;
Jingyao LIANG
;
Chao BI
;
Ridong YANG
;
Ping LI
;
Yanhua LIANG
;
Xibao ZHANG
;
Wenling CAO
- Keywords:
Neisseria gonorrhoeae;
azithromycin;
antimicrobial resistance;
NG-MAST
- From:
International Journal of Laboratory Medicine
2017;38(11):1495-1498,1501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the characteristics of genotyping and gene polymorphism of Neisseria gonorrhoeae(N.go) with azithromycin(AZM)-resistance(AZM-R) and decreased susceptibility to ceftriaxone(CROD).Methods The minimum inhibitory concentration(MIC) of AZM and CRO were determined.AZM-R isolates were detected for mutations in 23S rRNA,mtrR and penA genes.Genotypes were analyzed by using N.go multi-antigen sequence typing(NG-MAST).Results All total of 485 isolates of N.go were detected.77(15.9%) strains were AZM-R(MIC≥1 mg/L),including 33(6.8%) isolates of AZM low-level resistant(AZM-LLR,MIC=1 mg/L) strains and 44(9.1%) isolates of AZM middle-level resistant(AZM-MLR,MIC≥2 mg/L) strains.There were more CROD(MIC≥0.125 mg/L) strains in AZM-MLR isolates(43.2%),compared with those in AZM-LLR isolates(18.2%,P<0.05).The detected rates of 23S rRNA,mtrR,penA single or combined mutations were without significant differences between AZM-LLR isolates and AZM-MLR isolates(P>0.05).Similar results were found between combined AZM-LLR/CROD isolates and combined AZM-MLR/CROD isolates(P>0.05).No mutation of A2059G and AZM high-level resistant(AZM-HLR,MIC≥256 mg/L) isolate were found.Among 77 AZM-R isolates,67 sequence types(ST) were identified by NG-MAST,of which 30 types were novel.Most ST were represented by a single isolate.Conclusion AZM-R and CROD isolates,presented in this area,might be deserved continuous surveillance to identify the mechanism of concurrent resistance.
