Effects of arsenic trioxide and paclitaxel on the expression level of MTA1 in Caco-2 cells
10.3969/j.issn.1673-4130.2017.11.008
- VernacularTitle:三氧化二砷及紫杉醇对人结肠癌细胞MTA1 mRNA表达水平影响研究
- Author:
Youzhuo LIANG
;
Canfeng CHEN
- Keywords:
arsenic trioxide;
paclitaxel;
colon cancer;
Caco-2 cells;
MTA1
- From:
International Journal of Laboratory Medicine
2017;38(11):1461-1463,1467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of arsenic trioxide and paclitaxel on the expression level of metastasis-associated gene 1(MTA1) mRNA in Caco-2 cells.Methods Methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay was performed to detect the inhibition rate of different concentrations of arsenic trioxide and paclitaxel on the growth of Caco-2 cells,reverse transcription real-time polymerase chain reaction was performed to detect the expression level of MTA1 mRNA.Results With the increasing of the concentrations of arsenic trioxide and paclitaxel,the inhibition rate increased,while the expression level of MTA1 mRNA decreased.The inhibition rate was negatively correlated with the expression levle of MTA1 mRNA(r=-0.636,P<0.05).Conclusion The effects of arsenic trioxide and paclitaxel on the growth of Caco-2 cells and the expression level of MTA1 mRNA could be with dosage-dependence,and the inhibition effects might be negatively correlated with the expression level of MTA1 mRNA.
