Application value of RT-PCR in Mycoplasma pneumoniae infection
10.3969/j.issn.1673-4130.2017.11.014
- VernacularTitle:RT-PCR检测在疑似肺炎支原体感染患者中的应用研究
- Author:
Miao HU
;
Yanfen MA
;
Xiangping LI
;
Yaqiong MA
;
Pingfa LI
- Keywords:
real-time fluorescent quantity;
polymerase chain reaction;
Mycoplasma pneumoniae;
DNA
- From:
International Journal of Laboratory Medicine
2017;38(11):1480-1481,1484
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinic significance of real-time fluorescent quantity polymerase chain reaction(RT-PCR) for detection of Mycoplasma pneumoniae(MP) DNA in patients suspected with MP infection.Methods A total of 1 402 samples,including serum,sputum,pleural fluid,nasopharyngeal swab,alveolar irrigating solution and bronchial irrigating solution,were detected for MP-DNA by using RT-PCR.Results The total positive rate all samples were 12.20%.The positive rate of serum was the lowest,which was 2.36%.The positive rates of sputum,alveolar irrigating solution and bronchial irrigating solutions were relatively high,which were 62.96%,77.08% and 88.71%,respectively.Conclusion RT-PCR could be fitted for the detection of MP-DAN in various samples,which could be effective method for the diagnosis of MP infection.
