Influence of bloody cervical cell samples on HPV genotyping detection
10.3969/j.issn.1673-4130.2017.11.021
- VernacularTitle:血性宫颈脱落细胞标本对HPV核酸分型检测结果影响的研究
- Author:
Yanmei GE
;
Hang YUANG
;
Suyi FAN
;
Yuan MAO
;
Peng CAO
- Keywords:
human papillomavirus;
flowcytometry fluorescence hybridization;
hemoglobin;
sample volume
- From:
International Journal of Laboratory Medicine
2017;38(11):1499-1501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of bloody cervical cell samples on human papillomavirus(HPV) genotyping detection using flowcytometry fluorescence hybridization kit.Methods According to the concentration of hemoglobin(Hb),cervical cell samples were divided into five groups,including group A with Hb of 0 g/L(10 cases),group B with 0 g/L100 g/L.In each group,50% samples were with initial positive results of HPV genotyping detection.Every sample was detected using sample volume of 50,100,200,500 and 1 000 μL different volumes for testing and analysis results.Results For all samples with initial negative results,five samples with initial positive results in group A and five samples with initial positive results,the detected results of different sample volume were without differences.In the 10 samples,with initial positive results of group C,D and E,there were four,four and nine samples respectively,were with false negative detected results of different sample volumes.Conclusion Bloody cervical cell samples could cause false negative results of HPV genotyping detection using flowcytometry fluorescence hybridization kit.It might be necessary to set optimal sample volume according to the concentration of Hb for ensuring the accuracy of genotyping detection.
