The study of the role of candesartan in radiosensitivity of nasopharyngeal carcinoma CNE1 cells
10.3969/j.issn.1006-5725.2017.08.005
- VernacularTitle:坎地沙坦提高乏氧鼻咽癌CNE1细胞辐射敏感性
- Author:
Ying LIU
;
Lanfang ZHANG
;
Tan CHEN
;
Jie LIN
;
Guozhu XIE
- Keywords:
Nasopharyngeal carcinoma;
Candesartan;
Radiosensitivity.
- From:
The Journal of Practical Medicine
2017;33(8):1212-1216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of candesartan (an angiotensin II receptor 1 antagonist,AT1R) in radioresistance of human nasopharyngeal carcinoma CNE1 cells.Methods Cell growth of CNE1 with or without candesartan treatment was measured in vitro by MTT method;radiosensitivity of CNE1 with or without candesartan treatment was tested under normoxic or hypoxic conditions by clone formation assay.The expression of hypoxia-induced factor 1α(HIF-1α)in CNE1 cells was analysed by western blotting.Results Candesartan did not significantly inhibit the growth of CNE 1 cells in both normoxic and hypoxic conditions.Candesartan also did not influence the radiosensitivity of CNE1 cells in normoxic condition;however,it significantly increased the radiosensitivity of CNE1 cells in hypoxic condition.The expression of hypoxia-induced factor 1 α (HIF-1 α)in hypoxic CNE1 cells was significantly inhibited by candesartan treatment.Conclusion Candesartan does not significantly influence the proliferation of CNE1 cells in both normoxic and bypoxic conditions but significantly enhances the radiosensitivity of hypoxic CNE1 cells,in which the mechanisn may be involved in its inhibiting HIF1α expression in hypoxic CNE1 cells.
