The effect of autophagy and crosstalk with apoptosis induced by Ultraviolet in A375
10.3969/j.issn.1006-5725.2017.08.008
- VernacularTitle:紫外线对黑色素瘤细胞A375自噬与凋亡诱导效应
- Author:
Tongshuai WANG
;
Chaopeng WANG
;
Meijuan ZHOU
- Keywords:
Cutaneous malignant melanoma;
Ultraviolet;
Autophagy;
Apoptosis
- From:
The Journal of Practical Medicine
2017;33(8):1223-1227
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the autophagy effect and the crosstalk with apoptosis by UV in A375 cells.Methods GFP-RFP-LC3 lentivirus were used to evaluate the effect of autophagy after being irradiated with UV of different doses (0、10、30、50 mJ/cm2).After being treated with 30 mJ/cm2 irradiation,the apoptosis rate of A375 with or without autophagy inducer was evaluated by annexin V-FITC/PI with flow cytometry.Western blot was used to distinguish the biomarker of autophagy (BECN,LC3) and apoptosis (Caspase 3,9).Results After being irradiated with 10 mJ/cm2 or 30 rnJ/cm2 UV,the autophagosome was observed at 6 h and rich at 9 h.However,the dots of autophagy had been abundant continually from 3 h with 50 mJ/cm2.The ability of inducing autophagy of UVB is stronger than UVA.UVA and UVB showed synergistic effect in autophagy with the dose of 30 mJ/cm2.The Caspase 3 and Caspase 9 proteins were downregulated after 30 mJ/cm2 UV irradiation with autophagy biomarkers increasing,whereas the apoptosis biomarkers were enriched with the inhibition of autophagy.Conclusions UV can induce autophagy with more significant effect of UVB.Autophagy paly protective role by delaying apoptosis after 30 mJ/cm2 UV irradiation in A375.
