Role of Caveolin-1 in glargine in anti-inflammation and glycemic metabolism improvement of type 2 diabetic mice
10.3969/j.issn.1006-5725.2017.08.014
- VernacularTitle:Caveolin-1在甘精胰岛素改善2型糖尿病小鼠糖代谢及骨骼肌炎症中的作用
- Author:
Hangya PENG
;
Haicheng LI
;
Shuo LIN
;
Wen ZENG
;
Chuwen LIN
;
Keyi LIN
;
Longyi ZENG
- Keywords:
Caveolin-1;
Skeletal muscle;
Type 2 diabetic mice;
TNF-α;
Glargine
- From:
The Journal of Practical Medicine
2017;33(8):1247-1249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of glargine in glucose metabolism improvement and antiinflammation of skeletal muscle in Caveolin-1 silenced type 2 diabetic mice.Methods Multiple low doses of streptozotocin (STZ) intraperitoneal injection and high-fat high-glucose (HFHG) were used to induce type 2 diabetic mice model.The mice were divided into normal control group (NC group) and type 2 diabetic model group (T group).Then according to virus injection and glargine treatment,T group were further divided into type 2 diabetes group (T2DM group),type 2 diabetes with insulin treatment group (insulin group),Caveolin-1 silenced with insulin treatment group (LV-CAV1 group),and scramble virus with insulin treatment group (LV-GFP group).Glucose metabolism was accessed by the fluctuation of blood glucose.TNF-α protein expression in skeletal muscle was detected by Western blot.Results The glycemic control of LV-CAV1 group needed more dosages of glargine (P ＜ 0.05).The expression of TNFαin skeletal muscle was elevated in LV-CAV 1 group than that in LV-GFP and insulin group (P ＜ 0.05).Conclusion The anti-inflammation function and glycemic metabolism improvement of glargine may be associated with the expression of Caveoin-1 in skeletal muscle.
