LC-MS based measurement of androgens and its clinical significance in diagnosis of Sj(o)gren's syndrome

VernacularTitle:基于液相色谱-质谱的雄激素检测及对干燥综合征的诊疗价值
Author: Haijiang TONG ; Hongbin TONG ; Xinchang WANG ; Xiaofang LI ; Jian YE ; Zhiliang XU
Keywords: Serum; Androgens; Liquid chromatography tandem mass spectrometry (LC-MS); Sj(o)gren′s syndrome (SS)
From: Chinese Journal of Primary Medicine and Pharmacy 2017;24(13):1950-1953,后插1
CountryChina
Language:Chinese
Abstract: Objective To evaluate the clinical value of 6 androgens in the serum of Sj?gren's syndrome (SS) measured by liquid chromatography tandem mass spectrometry (LC-MS).Methods 32 SS patients (SS group) and 25 healthy people (healthy group) were included in this study.6 androgens in the serum were analyzed by LC-MS after prepared.PCA,PLS-DA models and t-test were used to class differentiation of androgens between two groups.Results The results of PLS-DA showed that SS group and healthy group could be well classed by 6 androgens.The levels of testerone (T),dihydrotestosterone (DHT),dehydroepiandrosterone (DHEA),androsterone and DHEAS in SS group were significantly lower than those in healthy group (t=8.536,2.438,3.172,4.158,4.489,all P<0.05).The samples before or after menopause could be distinguished between the two groups by PLS-DA.Conclusion The significant difference of androgens was discovered between SS patients and healthy people via the measurement of 6 androgens in serum.It may arise a new idea for diagnosis and treatment of SS.