The efficacy of intra-carotid infusion with cold saline in rats with acute cerebral ischemia
10.3969/j.issn.1006-5725.2017.07.002
- VernacularTitle:颈内动脉局部灌注冷生理盐水对大鼠急性脑梗死的治疗效果
- Author:
Wei SONG
;
Yongming WU
;
Zhong JI
;
Yabin JI
;
Suyue PAN
- Keywords:
Ischemic stroke;
Cerebral local hypothermia;
Intracarotid infusion;
Neuroprotection
- From:
The Journal of Practical Medicine
2017;33(7):1025-1028
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the safety and therapeutic effect of intra-carotid infusion with cold sa line in rats with acute focal cerebral ischemia-reperfusion injury.Methods 60 rats were randomly divided into six groups:sham operated group,normal infusion group,stroke group,local hypothermic group,local normothermic group,and systemic infusion group.Brain infarct volume and cerebral water content were analyzed 48 h after ischemia.Neurological deficits were assessed using the mNSS 24 h and 48 h after infarction.Results In the local hypothermic group,brain temperature was reduced to 33 to 34 ℃ within 5 to 10 minutes,and this significantly low temperature maintained to nearly 60 minutes after infusion continued.Physiological variables were not significantly different among each time point (P ＞ 0.05).No significant morphological abnormality was found in brain sections stained with TTC and HE.Animals receiving local cold infusion significantly decreased infarct volume and brain water content compared to stroke group (P ＜ 0.05).Both 24 h and 48 h mNSS in local hypothermic group was significantly lower than those in other groups (P ＜ 0.05).Conclusions Intra-carotid infusion with cold saline can quickly and effectively reduce brain temperature and is a relatively safe cooling method.Local hypothermia significantly reduced brain infarct volume,decreased brain water content and improved neurological functional outcomes after brain ischemia.
