Manumycin-induced apoptosis in U937 human myeloid leukemia cells via blocking PI3K/Akt signaling pathway

VernacularTitle:手霉素通过阻断PI3K/Akt信号通路诱导U937白血病细胞凋亡
Author: Huiting HUANG ; Jingao LI ; Dingzhang XIAO ; Jing CHEN ; Miaorong SHE
Keywords: Manumycin; U937 cells; ROS; Apoptosis; PI3K/Akt signal pathway
From: The Journal of Practical Medicine 2017;33(7):1066-1069
CountryChina
Language:Chinese
Abstract: Objective To explore the effects of manumycin on U937 cells and its mechanisms.Methods Apoptosis was detected by flow cytometry using annexin V and propidium iodide (PI) after treatment with manumycin (2 μmol/L) for different time.The reactive oxygen species (ROS) was detected by flow cytometry using Reactive Oxygen Species Assay Kit.The expressions of PI3K,P-Akt and Akt were detected by Western blotting.Results Compared with the 0 h treatment,manumycin treatment resulted in apoptosis (P ＜ 0.05) and ROS generation (P ＜ 0.05) gradually in U937 cell lines.Furthermore,manumycin also inhibited the activation of phosphatidylinositol-3 kinase (PI3K)/Akt pathway.Moreover,NAC could decrease manumycin-induced ROS generation and inhibit the effects of manumycin on the PI3K/Akt pathway and protect U937 cells from apoptosis induced by manumycin.Conclusion Manumycin induces apoptosis in U937 human leukemia cells through the regulation of the PI3K/Akt pathway and ROS production plays a critical role in the progress.