Effect of silencing ACAT1 gene by RNA interference on proliferation, migration and invasion of human colon cancer cells
10.3969/j.issn.1006-5725.2017.07.015
- VernacularTitle:ACAT1基因干扰对人结肠癌细胞增殖及侵袭转移的影响
- Author:
Xin CHEN
;
Huiling LIANG
;
Ximing XU
;
Yiqiao ZHANG
;
Neng CHEN
;
Dandan LIN
;
Ling FENG
;
Yongbin ZHENG
- Keywords:
Colon neoplasm;
Acyl coenzyme A cholesterol acyltransferase 1;
Small interfering RNA
- From:
The Journal of Practical Medicine
2017;33(7):1074-1077
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of silencing ACAT1 gene on colon cancer cells proliferation,migration,invasion and colon cancer development by using the small interference RNA (siRNA) in colon cancer cell line HT-29.Methods Acyl coenzyme A cholesterol acyltransferase 1 (ACAT1) gene was silenced in HT-29 cell lines using Hiperfect transfection reagent.The expression level of ACAT1 was detected by real time PCR.CFSE and transwell assays were used to evaluate the effect of ACAT1 gene interfering on cells proliferation,mi gration and invasion.Result ACAT1 mRNA expression decreased obviously after siRNA interference.Compared with pre-transfection,proliferation,migration and invasion of colon cancer cells have been significantly inhibited (P ＜ 0.05).Conclusion ACAT1 gene interference reduced proliferation,migration and of invasion of HT29 cells,which provide a new potential target for colon cancer treatment.
