Expression and Effect of LncRNA-MIAT in Tumor Necrosis Factor-α Induced Endothelial Cell Inflammation
10.3969/j.issn.1000-3614.2017.06.018
- VernacularTitle:LncRNA-MIAT在肿瘤坏死因子α介导的血管内皮细胞炎症中的表达及作用
- Author:
Chenglong REN
;
Lu ZHANG
;
Xianfeng NING
;
Qing ZHAO
;
Shanglang CAI
;
Wenzhong ZHANG
- Keywords:
Ribonucleases;
Tumor necrosis factor alpha;
Cell adhesion molecule-1
- From:
Chinese Circulation Journal
2017;32(6):607-611
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the expression of long non-coding RNA myocardial infarction associated transcript (LncRNA-MIAT) in tumor necrosis factor-α (TNF-α) induced endothelial cells (ECs) inflammation in vitroand to study the impact of LncRNA-MIAT on inflammatory regulation. Methods: LncRNA-MIAT expression in ECs was induced by TNF-α at different time and concentration. Expressions of intercellular adhesion molecule-1 (ICAM-1) and LncRNA-MIAT in inflammatory ECs were examined by quantitative real time polymerase chain reaction (qRT-PCR) and Western blot analysis. Moreover, ECs was transfected by siRNAMIAT to observe the effect of LncRNA-MIAT knock-down on ICAM-1 expression. Results: LncRNA-MIAT expression showed the increasing trend by elevated time and concentration of TNF-stimulation. Compared with TNF-α stimulation at 0h, 6h and 12h, LncRNA-MIAT expressions were increased at 24h and 48h of TNF-αstimulation respectively, allP<0.05; compared with TNF-α concentration at 0ng/ml and 0.125ng/ml, LncRNA-MIAT expressions were elevated by TNF-α stimulation at 1.000ng/ml and 10.000ng/ml respectively, allP<0.05. With siRNAMIAT knock-down, TNF-α induced ICAM-1 protein expression was significantly reduced in ECs,P<0.05. Conclusion: LncRNA-MIAT might be involved in ECs inflammatory response and it may play a role to promote inflammation.
